10/04/2016
Application and selection of egg donation
Egg donors must be in accordance with the egg on the nyeb donation application procedures, need to go through a preliminary screening and three rounds of interviews (and our clinical Co ordinator, doctors and psychologists) as well as some test. Only donor applicants through all of these screening steps, in order to be listed as NYEB's egg donation candidates, and the recipient couples to match and meet the requirements of NYEB. NYEB's egg donation program is highly selective.
First step egg donation match:
When a couple decides to use the donated eggs, they can view the NYEB egg donor library online and select a candidate from the donor library. NYEB's egg donation Coordinator will contact the donor to confirm her availability when the recipient is determined to match the donor's initial donor egg matching. If an egg donor and recipient wish to agree, the donor will perform all the blood tests required by the United States FDA, which will eventually determine the availability of the donor.
Step 2, the egg donor from the super row of treatment:
Egg donors will be under the guidance of a doctor, his daily use or injection from the hormone, so that the cycle of her cycle and the recipient to synchronize or meet the needs of NYEB. Ovarian stimulation from the Super Ovulation, is the egg donor daily injections of gonadotropin to stimulate her ovaries have a number of follicular development and growth. In the process of ovarian stimulation, egg donors will be closely monitored by the clinic, and through blood tests and ultrasound, in order to ensure good ovarian response, and not lead to excessive stimulation. This requires an egg donor several times a return visit to the NYEB clinic or our local clinic. These monitors are scheduled to work in the early hours of the morning, in order to avoid interfering with the daily work of the donors.
The third step - the donor recipient's endometrial preparation:
One side of the egg recipient needs to have a good endometrial environment, especially if the endometrium needs at least 7mm thick, which is an important factor in deciding the success or failure of the egg cycle. At the same time, the recipient needs to be treated with estrogen and progesterone to prepare for the embryo to be prepared for the embryo. The preparation of recipient endometrium and embryo transfer is usually not a problem. But in rare cases, part of the receiver is difficult to achieve minimum thickness of endometrium may need special treatment, such as additional supplies of compound vitamin; some recipient autoimmunity may to use other drugs, in order to improve the success of embryo implantation rate.
The fourth step - the donor's trigger ovulation and transva**nal ultrasound eggs:
In order to evaluate the effect of ovarian stimulation and determine the timing of oocyte retrieval, it is necessary to use transva**nal ultrasound to monitor the size of follicles, and to cooperate with blood tests to check the E2 value (estrogen), to adjust the dosage of the drug. When two to three more than the diameter of follicles larger than 1.8cm, and the number of follicles above 1.4cm and E2 value, it can be injected with human chorionic gonadotropin (hCG), to promote follicular maturation. HCG hours after injection of 34-36 to take eggs. The most commonly used mode of egg is in the local anesthesia or general anesthesia cases, transva**nal B ultrasound guided, will take the egg needle through the va**na fornix, direct ovarian eggs are aspirated, and immediately under the microscope the egg to embryo bearing culture solution in a Petri dish, 37 C incubator culture. Donors need to take a day off to get the rest of the body to restore the body. Remote donors are required to come to our clinic or related clinics for 2 nights and 3 days to get the eggs ready and take the eggs (NYEB provides travel expenses).
Fifth step - in vitro fertilization of the egg - and the recipient's embryo transfer:
S***m or s***m recipient husband provided the time and the day of oocyte retrieval for the same day. Wash your hands before taking the essence, use ma********on to keep semen. The cup is sterile, taken when not to touch the cup and the cup edge. The semen was treated by the method of upstream or Percoll density gradient centrifugation. 3-4 hours after the treatment of s***m and eggs in the same culture dish, after 18 hours of co culture, can be observed under the microscope fertilization. If the s***m quality is too poor to be naturally fertilized, it must be forced to be fertilized by a micro injection method (the s***m of the reference egg). Fertilized eggs in vitro culture 48-72 hours can be developed to 8-16 cell stage embryos. At this time based on the patient's age, have been pregnant and no and the quality of the embryo, the number of embryos transferred, the number of extra embryos can be frozen. Embryo transfer generally does not require anesthesia. At present, more than third days after fertilization, we use 3-5 days after fertilization. Delayed embryo transfer time is the fifth day of the blastocyst stage, to in vitro conditions require higher, but delayed time to transplant more in line with the physiology of pregnancy, but also through natural selection to eliminate poor quality embryos, can improve the pregnancy rate, reducing the rate of multiple births.
The sixth step, the donor's examination and recipient's pregnancy test:
After the egg donor eggs to return to the NYEB clinic or related clinics to review, so as to ensure that the donor after ovarian stimulation to restore normal body. In the majority of cases, donors will not have any problem, take the egg after the 1-2 can return to normal. If the donor has any discomfort, the NYEB clinic or related clinic will pay close attention to the physical condition of the donor and keep in touch with her until the donor is fully recovered. The recipient in embryo transplantation after two weeks of pregnancy test, through blood test to measure the level of hCG. At present, using injection method to support luteal progesterone. If you are pregnant, you can use hCG to continue to add to the 10 week of pregnancy.
Re use of STEP7- egg donors:
Egg donor